Download MendeleyCrystal structure of putative geranyltranstransferase from Pseudomonas fluorescens PF-5 complexed with magnesium
Malashkevich, V.N., Toro, R., Sauder, J.M., Burley, S.K., Almo, S.C.To be published.
3LSN
Crystal structure of putative geranyltranstransferase from PSEUDOMONAS fluorescens PF-5 complexed with magnesium
- PDB DOI: https://doi.org/10.2210/pdb3LSN/pdb
- Classification: TRANSFERASE
- Organism(s): Pseudomonas protegens Pf-5
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2010-02-12 Released: 2010-03-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.35 Å
- R-Value Free: 0.201
- R-Value Work: 0.179
- R-Value Observed: 0.180
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.7 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Geranyltranstransferase | 304 | Pseudomonas protegens Pf-5 | Mutation(s): 0 Gene Names: PFL_5509 EC: 2.5.1.10 |  | |
UniProt | |||||
Find proteins for Q4K5A6 (Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)) Explore Q4K5A6 Go to UniProtKB: Q4K5A6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q4K5A6 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MG Query on MG | B [auth A], C [auth A], D [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.35 Å
- R-Value Free: 0.201
- R-Value Work: 0.179
- R-Value Observed: 0.180
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 84.182 | α = 90 |
| b = 47.875 | β = 120.78 |
| c = 75.982 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| PHASER | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| CBASS | data collection |
| HKL-2000 | data reduction |
Entry History
Deposition Data
- Released Date: 2010-03-02 Deposition Author(s): Malashkevich, V.N., Toro, R., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2010-03-02
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Refinement description, Version format compliance - Version 1.2: 2017-11-01
Changes: Refinement description - Version 1.3: 2018-11-21
Changes: Data collection, Structure summary - Version 1.4: 2021-02-10
Changes: Database references, Derived calculations, Structure summary - Version 1.5: 2023-09-06
Changes: Data collection, Database references, Refinement description - Version 1.6: 2023-11-22
Changes: Data collection - Version 1.7: 2024-11-27
Changes: Structure summary
