Download MendeleyTranslation initiation factor 4E in complex with (SP)-iPr-m7GppSpG mRNA 5' cap analog
Warminski, M., Nowak, E., Kubacka, D., Kowalska, J., Nowotny, M., Jemielity, J.To be published.
5M81
Translation initiation factor 4E in complex with (SP)-iPr-m7GppSpG mRNA 5' cap analog
- PDB DOI: https://doi.org/10.2210/pdb5M81/pdb
- Classification: TRANSLATION
- Organism(s): Mus musculus
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2016-10-28 Released: 2017-12-20
- Funding Organization(s): Ministry of Science and Higher Education
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.219
- R-Value Work: 0.177
- R-Value Observed: 0.178
Starting Model: experimental
View more details
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Eukaryotic translation initiation factor 4E | 190 | Mus musculus | Mutation(s): 0 Gene Names: Eif4e |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P63073 (Mus musculus) Explore P63073 Go to UniProtKB: P63073 | |||||
IMPC: MGI:95305 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P63073 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| 7L2 Query on 7L2 | E [auth A], G [auth B], J [auth C], K [auth D] | [[[(3~{a}~{R},4~{R},6~{R},6~{a}~{R})-4-(2-azanyl-7-methyl-6-oxidanylidene-1~{H}-purin-7-ium-9-yl)-2,2-dimethyl-3~{a},4,6,6~{a}-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methoxy-oxidanyl-phosphoryl]oxy-sulfanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate C24 H34 N10 O17 P3 S DSKLYZYSTSOTOM-RGGYBXOHSA-O |  | ||
| GOL Query on GOL | F [auth A], H [auth B], I [auth B] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.219
- R-Value Work: 0.177
- R-Value Observed: 0.178
- Space Group: P 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 38.1 | α = 88.77 |
| b = 38.12 | β = 95.82 |
| c = 147.24 | γ = 103.44 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2017-12-20 Deposition Author(s): Warminski, M., Nowak, E., Kowalska, J., Jemielity, J., Nowotny, M.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Ministry of Science and Higher Education | Poland | DI2012 024842 |
Revision History (Full details and data files)
- Version 1.0: 2017-12-20
Type: Initial release - Version 1.1: 2024-01-17
Changes: Advisory, Data collection, Database references, Refinement description
