3AJA | pdb_00003aja

Crystal Structure of MSMEG_6394

PDB DOI: https://doi.org/10.2210/pdb3AJA/pdb

Classification: HYDROLASE
Organism(s): Mycolicibacterium smegmatis MC2 155
Expression System: Escherichia coli
Mutation(s): No
Membrane Protein: Yes OPM

Deposited: 2010-05-27 Released: 2010-08-11
Deposition Author(s): Vivian, J.P., Scoble, J., Beddoe, T., Rossjohn, J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free:
0.250 (Depositor), 0.250 (DCC)
R-Value Work:
0.213 (Depositor), 0.210 (DCC)
R-Value Observed:
0.215 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Functional characterisation of a lipase essential for mycobacterial viability

Crellin, P.K., Vivian, J.P., Scoble, J., Chow, F.M., West, N.P., Brammananth, R., Proellocks, N.I., Shahine, A., Le Nours, J., Wilce, M.C.J., Britton, W.J., Coppel, R.L., Rossjohn, J., Beddoe, T.

To be published.

Macromolecule Content

Total Structure Weight: 64.3 kDa
Atom Count: 3,956
Modelled Residue Count: 524
Deposited Residue Count: 604
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Putative uncharacterized protein	A, B	302	Mycolicibacterium smegmatis MC2 155	Mutation(s): 0 Gene Names: MSMEG_6394 EC: 3.1.1 Membrane Entity: Yes
UniProt
Find proteins for A0R619 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) Explore A0R619 Go to UniProtKB: A0R619
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0R619
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free: 0.250 (Depositor), 0.250 (DCC)
R-Value Work: 0.213 (Depositor), 0.210 (DCC)
R-Value Observed: 0.215 (Depositor)

Space Group: I 4₁ 2 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 130.434	α = 90
b = 130.434	β = 90
c = 209.533	γ = 90

Software Package:

Software Name	Purpose
CrystalClear	data collection
SOLVE	phasing
REFMAC	refinement
MOSFLM	data reduction
SCALA	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-08-11

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2010-08-11
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2024-10-30
Changes: Data collection, Database references, Structure summary

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.