3A5U | pdb_00003a5u

Promiscuity and specificity in DNA binding to SSB: Insights from the structure of the Mycobacterium smegmatis SSB-ssDNA complex

PDB DOI: https://doi.org/10.2210/pdb3A5U/pdb
NAKB: 3A5U

Classification: DNA BINDING PROTEIN
Organism(s): Mycolicibacterium smegmatis MC2 155
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2009-08-12 Released: 2010-08-18
Deposition Author(s): Kaushal, P.S., Manjunath, G.P., Sekar, K., Muniyappa, K., Vijayan, M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free:
0.283 (Depositor), 0.280 (DCC)
R-Value Work:
0.238 (Depositor), 0.230 (DCC)
R-Value Observed:
0.240 (Depositor)

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Promiscuity and specificity in DNA binding to SSB: Insights from the structure of the Mycobacterium smegmatis SSB-ssDNA complex.

Kaushal, P.S., Manjunath, G.P., Sekar, K., Muniyappa, K., Vijayan, M.

To be published.

Macromolecule Content

Total Structure Weight: 37.01 kDa
Atom Count: 2,236
Modelled Residue Count: 261
Deposited Residue Count: 291
Unique protein chains: 1
Unique nucleic acid chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Single-stranded DNA-binding protein	A, B	130	Mycolicibacterium smegmatis MC2 155	Mutation(s): 0 Gene Names: ssb
UniProt
Find proteins for Q9AFI5 (Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)) Explore Q9AFI5 Go to UniProtKB: Q9AFI5
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9AFI5
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.283 (Depositor), 0.280 (DCC)
R-Value Work: 0.238 (Depositor), 0.230 (DCC)
R-Value Observed: 0.240 (Depositor)

Space Group: P 4₃ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 112.33	α = 90
b = 112.33	β = 90
c = 48.49	γ = 90

Software Package:

Software Name	Purpose
MAR345dtb	data collection
PHASER	phasing
REFMAC	refinement
MOSFLM	data reduction
SCALA	data scaling

Structure Validation

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Entry History

Deposition Data

Released Date: 2010-08-18

Deposition Author(s):

Manjunath, G.P.

Revision History (Full details and data files)

Version 1.0: 2010-08-18
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2023-11-01
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.