1SV6 | pdb_00001sv6

Crystal structure of 2-hydroxypentadienoic acid hydratase from Escherichia Coli

PDB DOI: https://doi.org/10.2210/pdb1SV6/pdb

Classification: LYASE
Organism(s): Escherichia coli
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2004-03-28 Released: 2004-06-22
Deposition Author(s): Fedorov, A.A., Fedorov, E.V., Sharp, A., Almo, S.C., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free:
0.267 (Depositor), 0.270 (DCC)
R-Value Work:
0.239 (Depositor), 0.250 (DCC)
R-Value Observed:
0.243 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal structure of 2-hydroxypentadienoic acid hydratase from Escherichia Coli

Fedorov, A.A., Fedorov, E.V., Sharp, A., Almo, S.C.

To be published.

Macromolecule Content

Total Structure Weight: 144.6 kDa
Atom Count: 9,890
Modelled Residue Count: 1,305
Deposited Residue Count: 1,345
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
2-keto-4-pentenoate hydratase	A, B, C, D, E	269	Escherichia coli	Mutation(s): 0 Gene Names: MHPD, B0350 EC: 4.2.1 (PDB Primary Data), 4.2.1.80 (UniProt)
UniProt
Find proteins for P77608 (Escherichia coli (strain K12)) Explore P77608 Go to UniProtKB: P77608
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P77608
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free: 0.267 (Depositor), 0.270 (DCC)
R-Value Work: 0.239 (Depositor), 0.250 (DCC)
R-Value Observed: 0.243 (Depositor)

Space Group: P 1 2₁ 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 55.222	α = 90
b = 121.507	β = 94.1
c = 111.796	γ = 90

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
SOLVE	phasing
CNS	refinement

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2004-06-22

Deposition Author(s):

New York SGX Research Center for Structural Genomics (NYSGXRC)

Revision History (Full details and data files)

Version 1.0: 2004-06-22
Type: Initial release
Version 1.1: 2008-04-30
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2021-02-03
Changes: Structure summary
Version 1.4: 2024-02-14
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.