1DPW

STRUCTURE OF HEN EGG-WHITE LYSOZYME IN COMPLEX WITH MPD

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.64 Å
  • R-Value Free: 0.222 
  • R-Value Work: 0.178 

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This is version 1.4 of the entry. See complete history. 


Literature

Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.

Weiss, M.S., Palm, G.J., Hilgenfeld, R.

(2000) Acta Crystallogr D Biol Crystallogr 56: 952-958

  • DOI: https://doi.org/10.1107/s0907444900006685
  • Primary Citation of Related Structures:  
    1DPW, 1DPX

  • PubMed Abstract: 

    Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.

    • Organizational Affiliation: 

    Institute of Molecular Biotechnology, Department of Structural Biology and Crystallography, PO Box 100813, D-07708 Jena, Germany. [email protected]


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
LYSOZYME129Gallus gallusMutation(s): 0 
EC: 3.2.1.17
UniProt
Find proteins for P00698 (Gallus gallus)
Explore P00698 
Go to UniProtKB:  P00698
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP00698
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.64 Å
  • R-Value Free: 0.222 
  • R-Value Work: 0.178 
  • Space Group: P 43 21 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 77.51α = 90
b = 77.51β = 90
c = 37.42γ = 90
Software Package:
Software NamePurpose
DENZOdata reduction
SCALEPACKdata scaling
REFMACrefinement

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2000-01-03
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2011-11-23
    Changes: Other
  • Version 1.4: 2024-11-20
    Changes: Data collection, Database references, Derived calculations, Structure summary