6BW6

Human GPT (DPAGT1) H129 variant in complex with tunicamycin

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	Glycos_transf_4	e6bw6C1	A: alpha bundles	X: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	H: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	T: Bacterial cell wall synthesis membrane enzyme MraY-related	F: Glycos_transf_4	ECOD (1.6)
C	KOG2788	e6bw6C2	A: a+b two layers	X: SSHS domain	H: DPAGT1 insertion domain (From Topology)	T: DPAGT1 insertion domain	F: KOG2788	ECOD (1.6)
A	Glycos_transf_4	e6bw6A1	A: alpha bundles	X: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	H: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	T: Bacterial cell wall synthesis membrane enzyme MraY-related	F: Glycos_transf_4	ECOD (1.6)
A	KOG2788	e6bw6A2	A: a+b two layers	X: SSHS domain	H: DPAGT1 insertion domain (From Topology)	T: DPAGT1 insertion domain	F: KOG2788	ECOD (1.6)
B	Glycos_transf_4	e6bw6B1	A: alpha bundles	X: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	H: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	T: Bacterial cell wall synthesis membrane enzyme MraY-related	F: Glycos_transf_4	ECOD (1.6)
B	KOG2788	e6bw6B2	A: a+b two layers	X: SSHS domain	H: DPAGT1 insertion domain (From Topology)	T: DPAGT1 insertion domain	F: KOG2788	ECOD (1.6)
D	Glycos_transf_4	e6bw6D1	A: alpha bundles	X: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	H: Bacterial cell wall synthesis membrane enzyme MraY-related (From Topology)	T: Bacterial cell wall synthesis membrane enzyme MraY-related	F: Glycos_transf_4	ECOD (1.6)
D	KOG2788	e6bw6D2	A: a+b two layers	X: SSHS domain	H: DPAGT1 insertion domain (From Topology)	T: DPAGT1 insertion domain	F: KOG2788	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00953	Glycosyl transferase family 4 (Glycos_transf_4)	Glycosyl transferase family 4	-	Family
A, B, C, D	PF21383	DPAGT1 insertion domain (DPAGT1_ins)	DPAGT1 insertion domain	DPAGT1 catalyses the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway. This is the insertion domain of eukaryotic DPAGT1, a feature absent in prokaryotic counterparts [1,2]. It referred to as CL9 ...	DPAGT1 catalyses the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway. This is the insertion domain of eukaryotic DPAGT1, a feature absent in prokaryotic counterparts [1,2]. It referred to as CL9 in [1]; it adopts a mixed alpha/beta fold and it forms part of the substrate recognition site in human DPAGT1 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase	glycosyltransferase activity transferase activity catalytic activity UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity phosphotransferase activity, for other substituted phosphate groups transferase activity, transferring phosphorus-containing groups cation binding ion binding binding metal ion binding small molecule binding identical protein binding protein binding UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity	carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein N-linked glycosylation protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein N-linked glycosylation protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process protein metabolic process macromolecule metabolic process protein modification process dolichol-linked oligosaccharide biosynthetic process protein N-linked glycosylation via asparagine nucleoside phosphate metabolic process nucleotide-sugar metabolic process nucleobase-containing compound metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process amino sugar metabolic process phosphate-containing compound metabolic process small molecule metabolic process UDP-N-acetylglucosamine metabolic process phosphorus metabolic process polyprenol metabolic process organic hydroxy compound metabolic process alcohol metabolic process dolichol metabolic process isoprenoid metabolic process lipid metabolic process	cellular anatomical structure membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle organelle subcompartment endomembrane system endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane cellular anatomical structure membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle organelle subcompartment endomembrane system endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR048439	DPAGT1, insertion domain	Domain
A, B, C, D	IPR033895	UDP-GlcNAc-dolichyl-phosphate GlcNAc phosphotransferase	Family
A, B, C, D	IPR000715	Glycosyl transferase, family 4	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: Q9H3H5	congenital myasthenic syndrome DPAGT1-CDG congenital myasthenic syndrome 13 congenital disorder of glycosylation colorectal cancer Malignant neoplasm of breast osteosarcoma peripheral neuropathy tuberculosis arthrogryposis heart disease intellectual disability ovarian cancer plasma cell myeloma gastric cancer congenital myasthenic syndrome DPAGT1-CDG congenital myasthenic syndrome 13 congenital disorder of glycosylation colorectal cancer Malignant neoplasm of breast osteosarcoma peripheral neuropathy tuberculosis arthrogryposis heart disease intellectual disability ovarian cancer plasma cell myeloma gastric cancer hereditary diffuse gastric adenocarcinoma lung cancer peripheral nervous system disease Sjogren syndrome colorectal carcinoma gastric neoplasm inborn errors of metabolism

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D	OPM	Transmembrane	Alpha-helical polytopic	Glycosyl transferase family 4	Transmembrane glycosyl transferase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, D	MemProtMD	Annotated as membrane protein by MemProtMD

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.