5T5I

TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, ORTHORHOMBIC FORM AT 1.9 A

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FwdA	e5t5iA1	A: beta sandwiches	X: Composite domain of metallo-dependent hydrolases (From Topology)	H: Composite domain of metallo-dependent hydrolases (From Topology)	T: Composite domain of metallo-dependent hydrolases	F: FwdA	ECOD (1.6)
A	Amidohydro_1_N	e5t5iA2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Amidohydro_1_N	ECOD (1.6)
G [auth I]	FwdA	e5t5iI1	A: beta sandwiches	X: Composite domain of metallo-dependent hydrolases (From Topology)	H: Composite domain of metallo-dependent hydrolases (From Topology)	T: Composite domain of metallo-dependent hydrolases	F: FwdA	ECOD (1.6)
G [auth I]	Amidohydro_1_N	e5t5iI2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Amidohydro_1_N	ECOD (1.6)
B	Molybdopterin_2nd,Molybdop_Fe4S4_1	e5t5iB2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: Molybdopterin_2nd,Molybdop_Fe4S4_1	ECOD (1.6)
B	Molybdopterin_1st_1	e5t5iB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: Molybdopterin_1st_1	ECOD (1.6)
H [auth J]	Molybdop_Fe4S4_1	e5t5iJ2	A: a+b two layers	X: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	H: Formate dehydrogenase/DMSO reductase, domain 1 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domain 1	F: Molybdop_Fe4S4_1	ECOD (1.6)
H [auth J]	Molybdopterin_2nd	e5t5iJ3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: Molybdopterin_2nd	ECOD (1.6)
H [auth J]	Molybdopterin_1st_1	e5t5iJ1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Formate dehydrogenase/DMSO reductase, domains 2 and 3 (From Topology)	T: Formate dehydrogenase/DMSO reductase, domains 2 and 3	F: Molybdopterin_1st_1	ECOD (1.6)
C	GXGXG	e5t5iC1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Alpha subunit of glutamate synthase-C (From Topology)	T: Alpha subunit of glutamate synthase-C	F: GXGXG	ECOD (1.6)
I [auth K]	GXGXG	e5t5iK1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Alpha subunit of glutamate synthase-C (From Topology)	T: Alpha subunit of glutamate synthase-C	F: GXGXG	ECOD (1.6)
F [auth G]	Fer4_21_1	e5t5iG1	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
L [auth P]	Fer4_21_1	e5t5iP1	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
D	Molydop_binding_1	e5t5iD1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: Molydop_binding_1	ECOD (1.6)
J [auth L]	Molydop_binding_1	e5t5iL1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: double psi	F: Molydop_binding_1	ECOD (1.6)
E [auth F]	Fer4_7	e5t5iF3	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_7	ECOD (1.6)
E [auth F]	Fer4_21_1	e5t5iF2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
K [auth N]	Fer4_7	e5t5iN3	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_7	ECOD (1.6)
K [auth N]	Fer4_21_1	e5t5iN2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D	2.40.40.20	Mainly Beta	Beta Barrel	Barwin-like endoglucanases		CATH (4.3.0)
J [auth L]	2.40.40.20	Mainly Beta	Beta Barrel	Barwin-like endoglucanases		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, G [auth I]	PF07969	Amidohydrolase family (Amidohydro_3)	Amidohydrolase family	-	Domain
B, H [auth J]	PF00384	Molybdopterin oxidoreductase (Molybdopterin)	Molybdopterin oxidoreductase	-	Family
F [auth G], L [auth P]	PF12838	4Fe-4S dicluster domain (Fer4_7)	4Fe-4S dicluster domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contai ...	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.	Domain
D, J [auth L]	PF01568	Molydopterin dinucleotide binding domain (Molydop_binding)	Molydopterin dinucleotide binding domain	This domain is found in various molybdopterin - containing oxidoreductases and tungsten formylmethanofuran dehydrogenase subunit d (FwdD) and molybdenum formylmethanofuran dehydrogenase subunit (FmdD); where the domain constitutes almost the entir ...	This domain is found in various molybdopterin - containing oxidoreductases and tungsten formylmethanofuran dehydrogenase subunit d (FwdD) and molybdenum formylmethanofuran dehydrogenase subunit (FmdD); where the domain constitutes almost the entire subunit. The formylmethanofuran dehydrogenase catalyses the first step in methane formation from CO2 in methanogenic archaea and has a molybdopterin dinucleotide cofactor [1]. This domain corresponds to the C-terminal domain IV in dimethyl sulfoxide (DMSO)reductase which interacts with the 2-amino pyrimidone ring of both molybdopterin guanine dinucleotide molecules [2].	Domain
E [auth F], K [auth N]	PF12838	4Fe-4S dicluster domain (Fer4_7)	4Fe-4S dicluster domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contai ...	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.	Domain
E [auth F], K [auth N]	PF00037	4Fe-4S binding domain (Fer4)	4Fe-4S binding domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, G [auth I]	Tungsten formylmethanofuran dehydrogenase subunit fwdA	hydrolase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cation binding ion binding binding metal ion binding small molecule binding	-	-
B, H [auth J]	Tungsten formylmethanofuran dehydrogenase subunit B	iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding formylmethanofuran dehydrogenase activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity	cellular respiration energy derivation by oxidation of organic compounds alkane biosynthetic process methane metabolic process generation of precursor metabolites and energy hydrocarbon metabolic process alkane metabolic process biosynthetic process energy derivation by oxidation of reduced inorganic compounds methanogenesis metabolic process cellular process hydrocarbon biosynthetic process anaerobic respiration	-
C, I [auth K]	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C	cation binding transition metal ion binding ion binding binding metal ion binding small molecule binding formylmethanofuran dehydrogenase activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity	cellular respiration energy derivation by oxidation of organic compounds alkane biosynthetic process methane metabolic process generation of precursor metabolites and energy hydrocarbon metabolic process alkane metabolic process biosynthetic process energy derivation by oxidation of reduced inorganic compounds methanogenesis metabolic process cellular process hydrocarbon biosynthetic process methanogenesis, from carbon dioxide cellular respiration energy derivation by oxidation of organic compounds alkane biosynthetic process methane metabolic process generation of precursor metabolites and energy hydrocarbon metabolic process alkane metabolic process biosynthetic process energy derivation by oxidation of reduced inorganic compounds methanogenesis metabolic process cellular process hydrocarbon biosynthetic process methanogenesis, from carbon dioxide anaerobic respiration	-
F [auth G], L [auth P]	Tungsten formylmethanofuran dehydrogenase subunit fwdG	oxidoreductase activity catalytic activity iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding	-	-
D, J [auth L]	Tungsten formylmethanofuran dehydrogenase subunit fwdD	oxidoreductase activity catalytic activity binding organic cyclic compound binding molybdopterin cofactor binding	-	-
E [auth F], K [auth N]	Tungsten formylmethanofuran dehydrogenase subunit fwdF	oxidoreductase activity catalytic activity iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, G [auth I]	IPR012027	Formylmethanofuran dehydrogenase, subunit A	Family
A, G [auth I]	IPR013108	Amidohydrolase 3	Domain
A, G [auth I]	IPR050378	Metallo-dependent Hydrolases Superfamily	Family
A, G [auth I]	IPR011059	Metal-dependent hydrolase, composite domain superfamily	Homologous Superfamily
A, G [auth I]	IPR053635	Metallo-dependent Hydrolases FwdA/FmdA	Family
A, G [auth I]	IPR032466	Metal-dependent hydrolase	Homologous Superfamily
B, H [auth J]	IPR006656	Molybdopterin oxidoreductase	Domain
B, H [auth J]	IPR050123	Prokaryotic molybdopterin-containing oxidoreductase	Family
B, H [auth J]	IPR016457	Formylmethanofuran dehydrogenase, subunit B	Family
C, I [auth K]	IPR036485	Glutamate synthase, alpha subunit, C-terminal domain superfamily	Homologous Superfamily
C, I [auth K]	IPR054942	Tungsten-containing formylmethanofuran dehydrogenase subunit FwdC	Family
C, I [auth K]	IPR017550	Formylmethanofuran dehydrogenase subunit C	Domain
F [auth G], L [auth P]	IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	Conserved Site
F [auth G], L [auth P]	IPR050572	Iron-Sulfur Ferredoxin	Family
F [auth G], L [auth P]	IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	Domain
D, J [auth L]	IPR041717	FwdD/FmdC, molybdopterin dinucleotide-binding domain	Domain
D, J [auth L]	IPR053637	Archaeal lipid biosynthesis protein FwdD	Family
D, J [auth L]	IPR006657	Molybdopterin dinucleotide-binding domain	Domain
D, J [auth L]	IPR009010	Aspartate decarboxylase-like domain superfamily	Homologous Superfamily
D, J [auth L]	IPR012040	Formylmethanofuran dehydrogenase, subunit D	Family
E [auth F], K [auth N]	IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	Conserved Site
E [auth F], K [auth N]	IPR052977	Polyferredoxin-like electron transfer protein	Family
E [auth F], K [auth N]	IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	Domain
E [auth F], K [auth N]	IPR043256	Polyferredoxin MvhB-like	Family
E [auth F], K [auth N]	IPR053559	Polyferredoxin	Family