5H8L

Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) C158S mutant in complex with putrescine

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d5h8la_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
B	d5h8lb_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
C	d5h8lc_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
D	d5h8ld_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
E	d5h8le_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
F	d5h8lf_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
G	d5h8lg_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
H	d5h8lh_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
I	d5h8li_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
J	d5h8lj_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
K	d5h8lk_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
L	d5h8ll_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
M	d5h8lm_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
N	d5h8ln_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
O	d5h8lo_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
P	d5h8lp_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
M	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
N	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
O	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
P	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CN_hydrolase	e5h8lA1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
B	CN_hydrolase	e5h8lB1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
C	CN_hydrolase	e5h8lC1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
D	CN_hydrolase	e5h8lD1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
E	CN_hydrolase	e5h8lE1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
F	CN_hydrolase	e5h8lF1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
G	CN_hydrolase	e5h8lG1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
H	CN_hydrolase	e5h8lH1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
I	CN_hydrolase	e5h8lI1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
J	CN_hydrolase	e5h8lJ1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
K	CN_hydrolase	e5h8lK1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
L	CN_hydrolase	e5h8lL1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
M	CN_hydrolase	e5h8lM1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
N	CN_hydrolase	e5h8lN1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
O	CN_hydrolase	e5h8lO1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
P	CN_hydrolase	e5h8lP1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
B	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
C	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
D	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
E	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
F	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
G	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
H	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
I	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
J	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
K	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
L	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
M	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
N	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
O	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
P	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	PF00795	Carbon-nitrogen hydrolase (CN_hydrolase)	Carbon-nitrogen hydrolase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	N-carbamoylputrescine amidohydrolase	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides hydrolase activity N-carbamoylputrescine amidase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process polyamine metabolic process alpha-amino acid metabolic process arginine metabolic process putrescine biosynthetic process amine metabolic process biogenic amine biosynthetic process biosynthetic process amine biosynthetic process polyamine biosynthetic process putrescine biosynthetic process from arginine oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process polyamine metabolic process alpha-amino acid metabolic process arginine metabolic process putrescine biosynthetic process amine metabolic process biogenic amine biosynthetic process biosynthetic process amine biosynthetic process polyamine biosynthetic process putrescine biosynthetic process from arginine putrescine metabolic process metabolic process cellular process small molecule metabolic process biogenic amine metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR036526	Carbon-nitrogen hydrolase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR017755	N-carbamoylputrescine amidase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR050345	Aliphatic Amidase/Beta-Ureidopropionase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR003010	Carbon-nitrogen hydrolase	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.