3MI6

Crystal structure of the alpha-galactosidase from Lactobacillus brevis, Northeast Structural Genomics Consortium Target LbR11.

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8093325	3001528	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Galactose mutarotase-like	8093326	3000035	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	(Trans)glycosidases	8093327	3000313	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	(Trans)glycosidases	8093327	3000313	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8093325	3001528	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galactose mutarotase-like	8093326	3000035	SCOP2B (2022-06-29)
B	SCOP2 Family	GH36 C-terminal domain-like	8046283	4004999	SCOP2 (2022-06-29)
B	SCOP2 Family	GH36 N-terminal domain-like	8046286	4005000	SCOP2 (2022-06-29)
B	SCOP2 Family	Melibase catalytic domain-like	8049800	4006645	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Glycosyl hydrolase domain-like	8093325	3001528	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Galactose mutarotase-like	8093326	3000035	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	(Trans)glycosidases	8093327	3000313	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8093325	3001528	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Galactose mutarotase-like	8093326	3000035	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	(Trans)glycosidases	8093327	3000313	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	Glyco_hydro_36C	e3mi6C1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_36C	ECOD (1.6)
C	Glyco_hydro_36N	e3mi6C2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_36N	ECOD (1.6)
C	Melibiase	e3mi6C3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Melibiase	ECOD (1.6)
A	Glyco_hydro_36C	e3mi6A3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_36C	ECOD (1.6)
A	Glyco_hydro_36N	e3mi6A2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_36N	ECOD (1.6)
A	Melibiase	e3mi6A1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Melibiase	ECOD (1.6)
B	Glyco_hydro_36C	e3mi6B1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_36C	ECOD (1.6)
B	Glyco_hydro_36N	e3mi6B2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_36N	ECOD (1.6)
B	Melibiase	e3mi6B3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Melibiase	ECOD (1.6)
D	Glyco_hydro_36C	e3mi6D1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_36C	ECOD (1.6)
D	Glyco_hydro_36N	e3mi6D2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_36N	ECOD (1.6)
D	Melibiase	e3mi6D3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Melibiase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	2.70.98.60	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
C	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
C	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
A	2.70.98.60	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
A	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
B	2.70.98.60	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
B	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
B	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
D	2.70.98.60	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
D	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
D	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF02065	Melibiase (Melibiase)	Melibiase	-	Family
A, B, C, D	PF16875	Glycosyl hydrolase family 36 N-terminal domain (Glyco_hydro_36N)	Glycosyl hydrolase family 36 N-terminal domain	This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold [1].	Domain
A, B, C, D	PF16874	Glycosyl hydrolase family 36 C-terminal domain (Glyco_hydro_36C)	Glycosyl hydrolase family 36 C-terminal domain	This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Alpha-galactosidase	alpha-galactosidase activity hydrolase activity, acting on glycosyl bonds hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity galactosidase activity	carbohydrate metabolic process catabolic process carbohydrate catabolic process primary metabolic process metabolic process cellular process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR000111	Glycoside hydrolase family 27/36, conserved site	Conserved Site
A, B, C, D	IPR038417	Alpha-galactosidase, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D	IPR031704	Glycosyl hydrolase family 36, N-terminal	Domain
A, B, C, D	IPR031705	Glycosyl hydrolase family 36, C-terminal	Domain
A, B, C, D	IPR050985	Alpha-glycosidase and related enzymes	Family
A, B, C, D	IPR002252	Glycoside hydrolase family 36	Family
A, B, C, D	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A, B, C, D	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A, B, C, D	IPR013780	Glycosyl hydrolase, all-beta	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.