2V7G

Crystal Structure of an Engineered Urocanase Tetramer

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d2v7gb_	Multi-domain proteins (alpha and beta)	Urocanase	Urocanase	Urocanase	automated matches	(Pseudomonas putida ) [TaxId: 303 ],	SCOPe (2.08)
A	d2v7ga_	Multi-domain proteins (alpha and beta)	Urocanase	Urocanase	Urocanase	automated matches	(Pseudomonas putida ) [TaxId: 303 ],	SCOPe (2.08)
C	d2v7gc_	Multi-domain proteins (alpha and beta)	Urocanase	Urocanase	Urocanase	automated matches	(Pseudomonas putida ) [TaxId: 303 ],	SCOPe (2.08)
D	d2v7gd_	Multi-domain proteins (alpha and beta)	Urocanase	Urocanase	Urocanase	automated matches	(Pseudomonas putida ) [TaxId: 303 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Urocanase coenzyme-binding domain-like	8003579	3000075	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Urocanase coenzyme-binding domain-like	8003579	3000075	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Urocanase coenzyme-binding domain-like	8003579	3000075	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Urocanase coenzyme-binding domain-like	8003579	3000075	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Urocanase catalytic domain-like	8091450	3002512	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Urocanase_C	e2v7gB2	A: a+b two layers	X: Glutamine synthetase-like	H: C-terminal domain in urocanase (From Topology)	T: C-terminal domain in urocanase	F: Urocanase_C	ECOD (1.6)
B	Urocanase_N	e2v7gB1	A: a+b two layers	X: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	H: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	T: N-terminal a+b domain in urocanase (Pfam 01175)	F: Urocanase_N	ECOD (1.6)
B	Urocanase	e2v7gB3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Urocanase	ECOD (1.6)
A	Urocanase_C	e2v7gA2	A: a+b two layers	X: Glutamine synthetase-like	H: C-terminal domain in urocanase (From Topology)	T: C-terminal domain in urocanase	F: Urocanase_C	ECOD (1.6)
A	Urocanase_N	e2v7gA1	A: a+b two layers	X: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	H: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	T: N-terminal a+b domain in urocanase (Pfam 01175)	F: Urocanase_N	ECOD (1.6)
A	Urocanase	e2v7gA3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Urocanase	ECOD (1.6)
C	Urocanase_C	e2v7gC2	A: a+b two layers	X: Glutamine synthetase-like	H: C-terminal domain in urocanase (From Topology)	T: C-terminal domain in urocanase	F: Urocanase_C	ECOD (1.6)
C	Urocanase_N	e2v7gC1	A: a+b two layers	X: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	H: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	T: N-terminal a+b domain in urocanase (Pfam 01175)	F: Urocanase_N	ECOD (1.6)
C	Urocanase	e2v7gC3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Urocanase	ECOD (1.6)
D	Urocanase_C	e2v7gD2	A: a+b two layers	X: Glutamine synthetase-like	H: C-terminal domain in urocanase (From Topology)	T: C-terminal domain in urocanase	F: Urocanase_C	ECOD (1.6)
D	Urocanase_N	e2v7gD1	A: a+b two layers	X: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	H: N-terminal a+b domain in urocanase (Pfam 01175) (From Topology)	T: N-terminal a+b domain in urocanase (Pfam 01175)	F: Urocanase_N	ECOD (1.6)
D	Urocanase	e2v7gD3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: Urocanase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.1770.10	Alpha Beta	3-Layer(aba) Sandwich	Urocanase fold	Urocanase superfamily	CATH (4.3.0)
B	3.40.50.10730	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Urocanase like domains	CATH (4.3.0)
A	3.40.1770.10	Alpha Beta	3-Layer(aba) Sandwich	Urocanase fold	Urocanase superfamily	CATH (4.3.0)
A	3.40.50.10730	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Urocanase like domains	CATH (4.3.0)
C	3.40.1770.10	Alpha Beta	3-Layer(aba) Sandwich	Urocanase fold	Urocanase superfamily	CATH (4.3.0)
C	3.40.50.10730	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Urocanase like domains	CATH (4.3.0)
D	3.40.1770.10	Alpha Beta	3-Layer(aba) Sandwich	Urocanase fold	Urocanase superfamily	CATH (4.3.0)
D	3.40.50.10730	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Urocanase like domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF17392	Urocanase C-terminal domain (Urocanase_C)	Urocanase C-terminal domain	-	Domain
A, B, C, D	PF01175	Urocanase Rossmann-like domain (Urocanase)	Urocanase Rossmann-like domain	-	Domain
A, B, C, D	PF17391	Urocanase N-terminal domain (Urocanase_N)	Urocanase N-terminal domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	UROCANATE HYDRATASE	urocanate hydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity	catabolic process oxoacid metabolic process aromatic amino acid family catabolic process amino acid metabolic process organic acid metabolic process organic acid catabolic process imidazole-containing compound metabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process catabolic process oxoacid metabolic process aromatic amino acid family catabolic process amino acid metabolic process organic acid metabolic process organic acid catabolic process imidazole-containing compound metabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process aromatic amino acid metabolic process L-histidine catabolic process to glutamate and formate L-histidine metabolic process imidazole-containing compound catabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process L-amino acid catabolic process primary metabolic process L-amino acid metabolic process proteinogenic amino acid metabolic process L-histidine catabolic process alpha-amino acid catabolic process proteinogenic amino acid catabolic process metabolic process cellular process monocarboxylic acid metabolic process small molecule catabolic process formate metabolic process L-histidine catabolic process to glutamate and formamide amide catabolic process amide metabolic process formamide metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR035400	Urocanase, N-terminal domain	Domain
A, B, C, D	IPR036190	Urocanase superfamily	Homologous Superfamily
A, B, C, D	IPR035085	Urocanase, Rossmann-like domain	Domain
A, B, C, D	IPR023636	Urocanase conserved site	Conserved Site
A, B, C, D	IPR038364	Urocanase, central domain superfamily	Homologous Superfamily
A, B, C, D	IPR023637	Urocanase-like	Family
A, B, C, D	IPR035401	Urocanase, C-terminal domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.