5H8I

Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) in complex with N-(dihydroxymethyl)putrescine

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d5h8ia_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
B	d5h8ib_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
C	d5h8ic_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
D	d5h8id_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
E	d5h8ie_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
F	d5h8if_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
G	d5h8ig_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
H	d5h8ih_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
I	d5h8ii_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
J	d5h8ij_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
K	d5h8ik_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
L	d5h8il_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
M	d5h8im_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
N	d5h8in_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
O	d5h8io_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)
P	d5h8ip_	Alpha and beta proteins (a+b)	Carbon-nitrogen hydrolase	Carbon-nitrogen hydrolase	automated matches	automated matches	Barrel medic (Medicago truncatula ) [TaxId: 3880 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
H	SCOP2 Family	Carbon-nitrogen hydrolases-like	8081721	4002312	SCOP2 (2022-06-29)
H	SCOP2 Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2 (2022-06-29)
I	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
M	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
N	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
O	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)
P	SCOP2B Superfamily	Carbon-nitrogen hydrolases	8081722	3001086	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	CN_hydrolase	e5h8iA1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
B	CN_hydrolase	e5h8iB1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
C	CN_hydrolase	e5h8iC1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
D	CN_hydrolase	e5h8iD1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
E	CN_hydrolase	e5h8iE1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
F	CN_hydrolase	e5h8iF1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
G	CN_hydrolase	e5h8iG1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
H	CN_hydrolase	e5h8iH1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
I	CN_hydrolase	e5h8iI1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
J	CN_hydrolase	e5h8iJ1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
K	CN_hydrolase	e5h8iK1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
L	CN_hydrolase	e5h8iL1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
M	CN_hydrolase	e5h8iM1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
N	CN_hydrolase	e5h8iN1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
O	CN_hydrolase	e5h8iO1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)
P	CN_hydrolase	e5h8iP1	A: a+b four layers	X: Carbon-nitrogen hydrolase-like	H: Carbon-nitrogen hydrolase (From Topology)	T: Carbon-nitrogen hydrolase	F: CN_hydrolase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
B	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
C	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
D	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
E	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
F	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
G	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
H	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
I	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
J	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
K	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
L	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
M	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
N	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
O	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)
P	3.60.110.10	Alpha Beta	4-Layer Sandwich	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase	Carbon-nitrogen hydrolase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	PF00795	Carbon-nitrogen hydrolase (CN_hydrolase)	Carbon-nitrogen hydrolase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	N-carbamoylputrescine amidohydrolase	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides hydrolase activity N-carbamoylputrescine amidase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process polyamine metabolic process alpha-amino acid metabolic process arginine metabolic process putrescine biosynthetic process amine metabolic process biogenic amine biosynthetic process biosynthetic process amine biosynthetic process polyamine biosynthetic process putrescine biosynthetic process from arginine oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process polyamine metabolic process alpha-amino acid metabolic process arginine metabolic process putrescine biosynthetic process amine metabolic process biogenic amine biosynthetic process biosynthetic process amine biosynthetic process polyamine biosynthetic process putrescine biosynthetic process from arginine putrescine metabolic process metabolic process cellular process small molecule metabolic process biogenic amine metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR036526	Carbon-nitrogen hydrolase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR017755	N-carbamoylputrescine amidase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR050345	Aliphatic Amidase/Beta-Ureidopropionase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P	IPR003010	Carbon-nitrogen hydrolase	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.