4RET

Crystal structure of the Na,K-ATPase E2P-digoxin complex with bound magnesium

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D [auth C]	E1-E2_ATPase_N,ATPase-IIC_X-K	e4retC1	A: beta sandwiches	X: jelly-roll	H: Calcium ATPase, transduction domain A (From Topology)	T: Calcium ATPase, transduction domain A	F: E1-E2_ATPase_N,ATPase-IIC_X-K	ECOD (1.6)
D [auth C]	Cation_ATPase_C	e4retC4	A: alpha bundles	X: Calcium ATPase transmembrane domain-related (From Homology)	H: Calcium ATPase transmembrane domain-related	T: Calcium ATPase transmembrane domain M	F: Cation_ATPase_C	ECOD (1.6)
D [auth C]	Cation_ATPase	e4retC2	A: a+b complex topology	X: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	H: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	T: Metal cation-transporting ATPase, ATP-binding domain	F: Cation_ATPase	ECOD (1.6)
D [auth C]	Hydrolase_3_1	e4retC3	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: HAD-like	F: Hydrolase_3_1	ECOD (1.6)
A	E1-E2_ATPase_N,ATPase-IIC_X-K	e4retA2	A: beta sandwiches	X: jelly-roll	H: Calcium ATPase, transduction domain A (From Topology)	T: Calcium ATPase, transduction domain A	F: E1-E2_ATPase_N,ATPase-IIC_X-K	ECOD (1.6)
A	Cation_ATPase_C	e4retA4	A: alpha bundles	X: Calcium ATPase transmembrane domain-related (From Homology)	H: Calcium ATPase transmembrane domain-related	T: Calcium ATPase transmembrane domain M	F: Cation_ATPase_C	ECOD (1.6)
A	Cation_ATPase	e4retA3	A: a+b complex topology	X: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	H: Metal cation-transporting ATPase, ATP-binding domain (From Topology)	T: Metal cation-transporting ATPase, ATP-binding domain	F: Cation_ATPase	ECOD (1.6)
A	Hydrolase_3_1	e4retA1	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: HAD-like	F: Hydrolase_3_1	ECOD (1.6)
E [auth D]	Na_K-ATPase_C	e4retD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Na_K-ATPase_C	ECOD (1.6)
E [auth D]	Na_K-ATPase_N	e4retD2	A: extended segments	X: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	H: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	T: Transmembrane helix in sodium-potassium ATPase beta subunit	F: Na_K-ATPase_N	ECOD (1.6)
B	Na_K-ATPase_C	e4retB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Na_K-ATPase_C	ECOD (1.6)
B	Na_K-ATPase_N	e4retB2	A: extended segments	X: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	H: Transmembrane helix in sodium-potassium ATPase beta subunit (From Topology)	T: Transmembrane helix in sodium-potassium ATPase beta subunit	F: Na_K-ATPase_N	ECOD (1.6)
C [auth G]	ATP1G1_PLM_MAT8	e4retG1	A: extended segments	X: FXYD family (From Topology)	H: FXYD family (From Topology)	T: FXYD family	F: ATP1G1_PLM_MAT8	ECOD (1.6)
F [auth E]	ATP1G1_PLM_MAT8	e4retE1	A: extended segments	X: FXYD family (From Topology)	H: FXYD family (From Topology)	T: FXYD family	F: ATP1G1_PLM_MAT8	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D [auth C]	2.70.150.10	Mainly Beta	Distorted Sandwich	Calcium-transporting ATPase, cytoplasmic transduction domain A	Calcium-transporting ATPase, cytoplasmic transduction domain A	CATH (4.3.0)
D [auth C]	1.20.1110.10	Mainly Alpha	Up-down Bundle	Calcium-transporting ATPase, transmembrane domain	Calcium-transporting ATPase, transmembrane domain	CATH (4.3.0)
D [auth C]	3.40.50.1000	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	HAD superfamily/HAD-like	CATH (4.3.0)
D [auth C]	3.40.1110.10	Alpha Beta	3-Layer(aba) Sandwich	Calcium-transporting ATPase, cytoplasmic domain N	Calcium-transporting ATPase, cytoplasmic domain N	CATH (4.3.0)
A	2.70.150.10	Mainly Beta	Distorted Sandwich	Calcium-transporting ATPase, cytoplasmic transduction domain A	Calcium-transporting ATPase, cytoplasmic transduction domain A	CATH (4.3.0)
A	1.20.1110.10	Mainly Alpha	Up-down Bundle	Calcium-transporting ATPase, transmembrane domain	Calcium-transporting ATPase, transmembrane domain	CATH (4.3.0)
A	3.40.50.1000	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	HAD superfamily/HAD-like	CATH (4.3.0)
A	3.40.1110.10	Alpha Beta	3-Layer(aba) Sandwich	Calcium-transporting ATPase, cytoplasmic domain N	Calcium-transporting ATPase, cytoplasmic domain N	CATH (4.3.0)
E [auth D]	1.20.5.170	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
E [auth D]	2.60.40.1660	Mainly Beta	Sandwich	Immunoglobulin-like	Na, k-atpase alpha subunit.	CATH (4.3.0)
B	2.60.40.1660	Mainly Beta	Sandwich	Immunoglobulin-like	Na, k-atpase alpha subunit.	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D [auth C]	PF00689	Cation transporting ATPase, C-terminus (Cation_ATPase_C)	Cation transporting ATPase, C-terminus	-	Family
A, D [auth C]	PF00702	haloacid dehalogenase-like hydrolase (Hydrolase)	haloacid dehalogenase-like hydrolase	This family is structurally different from the alpha/beta hydrolase family (Pfam:PF00561). This family includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. The structure of the family consists of two domains. One is an inserted fo ...	This family is structurally different from the alpha/beta hydrolase family (Pfam:PF00561). This family includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. The structure of the family consists of two domains. One is an inserted four helix bundle, which is the least well conserved region of the alignment, between residues 16 and 96 of Swiss:P24069. The rest of the fold is composed of the core alpha/beta domain [1]. Those members with the characteristic DxD triad at the N-terminus are probably phosphatidylglycerolphosphate (PGP) phosphatases involved in cardiolipin biosynthesis in the mitochondria [2].	Domain
A, D [auth C]	PF00690	Cation transporter/ATPase, N-terminus (Cation_ATPase_N)	Cation transporter/ATPase, N-terminus	Members of this families are involved in Na+/K+, H+/K+, Ca++ and Mg++ transport.	Domain
A, D [auth C]	PF00122	E1-E2 ATPase (E1-E2_ATPase)	E1-E2 ATPase	-	Family
A, D [auth C]	PF13246	Cation transport ATPase (P-type) (Cation_ATPase)	Cation transport ATPase (P-type)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth C]	Sodium/potassium-transporting ATPase subunit alpha-1	cation binding ion binding binding potassium ion binding alkali metal ion binding metal ion binding small molecule binding monoatomic cation transmembrane transporter activity P-type transmembrane transporter activity P-type sodium transporter activity potassium ion transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity P-type sodium:potassium-exchanging transporter activity cation binding ion binding binding potassium ion binding alkali metal ion binding metal ion binding small molecule binding monoatomic cation transmembrane transporter activity P-type transmembrane transporter activity P-type sodium transporter activity potassium ion transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity P-type sodium:potassium-exchanging transporter activity ATPase-coupled monoatomic cation transmembrane transporter activity sodium ion transmembrane transporter activity ATP-dependent activity P-type potassium transmembrane transporter activity P-type ion transporter activity active monoatomic ion transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity metal ion transmembrane transporter activity transmembrane transporter activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity sodium ion binding ATPase binding protein binding enzyme binding	potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport cellular process monoatomic cation transport establishment or maintenance of transmembrane electrochemical gradient membrane repolarization biological regulation regulation of biological quality regulation of membrane potential sodium ion export across plasma membrane export from cell sodium ion transmembrane transport export across plasma membrane metal ion transport sodium ion transport import into cell import across plasma membrane potassium ion transmembrane transport inorganic cation import across plasma membrane inorganic ion import across plasma membrane potassium ion transport potassium ion import across plasma membrane regulation of localization regulation of biological process regulation of transport regulation of monoatomic ion transport regulation of sodium ion transport regulation of metal ion transport	cellular anatomical structure membrane membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle intracellular membrane-bounded organelle intracellular organelle melanosome cytoplasmic vesicle organelle cytoplasm vesicle cell periphery cellular anatomical structure membrane membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle intracellular membrane-bounded organelle intracellular organelle melanosome cytoplasmic vesicle organelle cytoplasm vesicle cell periphery sarcolemma plasma membrane basal part of cell basal plasma membrane plasma membrane region basolateral plasma membrane ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane protein complex sodium:potassium-exchanging ATPase complex transporter complex cation-transporting ATPase complex transmembrane transporter complex axon cell projection neuron projection plasma membrane bounded cell projection
B, E [auth D]	Sodium/potassium-transporting ATPase subunit beta-1	ATPase activator activity molecular function activator activity molecular function regulator activity ATPase regulator activity monoatomic cation transmembrane transporter activity P-type transmembrane transporter activity P-type sodium transporter activity potassium ion transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity P-type sodium:potassium-exchanging transporter activity ATPase-coupled monoatomic cation transmembrane transporter activity sodium ion transmembrane transporter activity ATP-dependent activity ATPase activator activity molecular function activator activity molecular function regulator activity ATPase regulator activity monoatomic cation transmembrane transporter activity P-type transmembrane transporter activity P-type sodium transporter activity potassium ion transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity P-type sodium:potassium-exchanging transporter activity ATPase-coupled monoatomic cation transmembrane transporter activity sodium ion transmembrane transporter activity ATP-dependent activity P-type potassium transmembrane transporter activity P-type ion transporter activity active monoatomic ion transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity metal ion transmembrane transporter activity transmembrane transporter activity protein-macromolecule adaptor activity molecular adaptor activity ATPase binding protein binding binding enzyme binding	potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis regulation of localization potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis regulation of localization positive regulation of cation transmembrane transport regulation of cellular process transport sodium ion export across plasma membrane monoatomic ion transmembrane transport positive regulation of biological process sodium ion transmembrane transport regulation of monoatomic ion transmembrane transport export across plasma membrane regulation of sodium ion transport metal ion transport regulation of sodium ion transmembrane transport positive regulation of sodium ion transport monoatomic cation transmembrane transport localization positive regulation of monoatomic ion transport positive regulation of sodium ion transmembrane transport positive regulation of transmembrane transport monoatomic ion transport positive regulation of monoatomic ion transmembrane transport monoatomic cation transport regulation of metal ion transport positive regulation of cellular process regulation of biological process regulation of transport regulation of monoatomic ion transport regulation of sodium ion export across plasma membrane biological regulation export from cell inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport regulation of transmembrane transport positive regulation of transport cellular process regulation of monoatomic cation transmembrane transport positive regulation of sodium ion export across plasma membrane sodium ion transport proton transmembrane transport establishment or maintenance of transmembrane electrochemical gradient cell adhesion membrane repolarization regulation of biological quality regulation of membrane potential import into cell positive regulation of potassium ion import across plasma membrane regulation of potassium ion transport potassium ion transmembrane transport potassium ion import across plasma membrane import across plasma membrane regulation of potassium ion transmembrane transport inorganic cation import across plasma membrane positive regulation of potassium ion transport positive regulation of potassium ion transmembrane transport inorganic ion import across plasma membrane potassium ion transport regulation of potassium ion import biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism response to stress innate immune response response to external biotic stimulus regulation of P-type sodium:potassium-exchanging transporter activity regulation of ATP-dependent activity positive regulation of ATP-dependent activity regulation of potassium ion transmembrane transporter activity regulation of molecular function positive regulation of P-type sodium:potassium-exchanging transporter activity positive regulation of sodium ion transmembrane transporter activity regulation of transmembrane transporter activity regulation of transporter activity positive regulation of molecular function regulation of sodium ion transmembrane transporter activity regulation of monoatomic ion transmembrane transporter activity positive regulation of transporter activity positive regulation of ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity	cell periphery cellular anatomical structure apical part of cell membrane plasma membrane plasma membrane region apical plasma membrane sarcolemma ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane protein complex sodium:potassium-exchanging ATPase complex transporter complex cell periphery cellular anatomical structure apical part of cell membrane plasma membrane plasma membrane region apical plasma membrane sarcolemma ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane protein complex sodium:potassium-exchanging ATPase complex transporter complex cation-transporting ATPase complex transmembrane transporter complex
C [auth G], F [auth E]	Na+/K+ ATPase gamma subunit transcript variant a	channel regulator activity ion channel regulator activity molecular function regulator activity transporter regulator activity ATPase activator activity molecular function activator activity ATPase regulator activity protein-macromolecule adaptor activity molecular adaptor activity	potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport potassium ion homeostasis inorganic ion homeostasis homeostatic process cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis intracellular potassium ion homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis sodium ion homeostasis intracellular sodium ion homeostasis transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport cellular process monoatomic cation transport establishment or maintenance of transmembrane electrochemical gradient sodium ion export across plasma membrane export from cell sodium ion transmembrane transport export across plasma membrane metal ion transport sodium ion transport import into cell import across plasma membrane potassium ion transmembrane transport inorganic cation import across plasma membrane inorganic ion import across plasma membrane potassium ion transport potassium ion import across plasma membrane regulation of P-type sodium:potassium-exchanging transporter activity regulation of ATP-dependent activity regulation of localization positive regulation of cation transmembrane transport regulation of cellular process positive regulation of ATP-dependent activity regulation of sodium ion transport regulation of sodium ion transmembrane transport positive regulation of sodium ion transport positive regulation of monoatomic ion transport positive regulation of monoatomic ion transmembrane transport regulation of potassium ion transmembrane transporter activity regulation of biological process regulation of monoatomic ion transport regulation of potassium ion transmembrane transport regulation of molecular function regulation of transmembrane transport positive regulation of P-type sodium:potassium-exchanging transporter activity regulation of potassium ion transport positive regulation of sodium ion transmembrane transporter activity positive regulation of biological process regulation of transmembrane transporter activity regulation of monoatomic ion transmembrane transport regulation of transporter activity positive regulation of molecular function regulation of sodium ion transmembrane transporter activity positive regulation of transmembrane transport positive regulation of sodium ion transmembrane transport regulation of metal ion transport positive regulation of cellular process regulation of monoatomic ion transmembrane transporter activity regulation of transport biological regulation positive regulation of potassium ion transport positive regulation of transport positive regulation of potassium ion transmembrane transport positive regulation of transporter activity positive regulation of ion transmembrane transporter activity regulation of monoatomic cation transmembrane transport positive regulation of potassium ion transmembrane transporter activity	ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane plasma membrane membrane protein complex sodium:potassium-exchanging ATPase complex transporter complex cation-transporting ATPase complex cell periphery cellular anatomical structure transmembrane transporter complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D [auth C]	IPR005775	P-type ATPase subfamily IIC, subunit alpha	Family
A, D [auth C]	IPR004014	Cation-transporting P-type ATPase, N-terminal	Domain
A, D [auth C]	IPR001757	P-type ATPase	Family
A, D [auth C]	IPR044492	P-type ATPase, haloacid dehalogenase domain	Domain
A, D [auth C]	IPR006068	Cation-transporting P-type ATPase, C-terminal	Domain
A, D [auth C]	IPR018303	P-type ATPase, phosphorylation site	PTM
A, D [auth C]	IPR023298	P-type ATPase, transmembrane domain superfamily	Homologous Superfamily
A, D [auth C]	IPR050510	Cation transport ATPase (P-type)	Family
A, D [auth C]	IPR023214	HAD superfamily	Homologous Superfamily
A, D [auth C]	IPR008250	P-type ATPase, A domain superfamily	Homologous Superfamily
A, D [auth C]	IPR036412	HAD-like superfamily	Homologous Superfamily
A, D [auth C]	IPR023299	P-type ATPase, cytoplasmic domain N	Homologous Superfamily
B, E [auth D]	IPR000402	Sodium/potassium-transporting ATPase subunit beta	Family
B, E [auth D]	IPR038702	Sodium/potassium-transporting ATPase subunit beta superfamily	Homologous Superfamily
C [auth G], F [auth E]	IPR047297	Ion-transport regulator, FXYD motif	Conserved Site
C [auth G], F [auth E]	IPR000272	Ion-transport regulator, FXYD family	Family
C [auth G], F [auth E]	IPR047282	Sodium/potassium-transporting ATPase subunit gamma	Family

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C [auth G], D [auth C], E [auth D] A, B, C [auth G], D [auth C], E [auth D], F [auth E]	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	P-type ATPase	Na,K-ATPase, phosphorylated form in complex with bufalin

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, D [auth C]	PHD	Parent Component: ASP RESID: AA0033 PSI-MOD : L-aspartic 4-phosphoric anhydride MOD:00042

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.