4A16

Structure of mouse Acetylcholinesterase complex with Huprine derivative

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4a16a1	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Acetylcholinesterase-like	automated matches	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d4a16a2	Artifacts	Tags	Tags	Tags	C-terminal Tags	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d4a16b1	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Acetylcholinesterase-like	automated matches	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d4a16b2	Artifacts	Tags	Tags	Tags	C-terminal Tags	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D	d4a16d1	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Acetylcholinesterase-like	automated matches	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D	d4a16d2	Artifacts	Tags	Tags	Tags	C-terminal Tags	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d4a16c1	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Acetylcholinesterase-like	automated matches	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d4a16c2	Artifacts	Tags	Tags	Tags	C-terminal Tags	HOUSE MOUSE (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	alpha/beta-Hydrolases	8042208	3000102	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	alpha/beta-Hydrolases	8042208	3000102	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	alpha/beta-Hydrolases	8042208	3000102	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	alpha/beta-Hydrolases	8042208	3000102	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	COesterase	e4a16A1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: COesterase	ECOD (1.6)
B	COesterase	e4a16B1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: COesterase	ECOD (1.6)
D	COesterase	e4a16D1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: COesterase	ECOD (1.6)
C	COesterase	e4a16C1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: COesterase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
B	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
D	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
C	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00135	Carboxylesterase family (COesterase)	Carboxylesterase family	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	ACETYLCHOLINESTERASE	acetylcholinesterase activity hydrolase activity, acting on ester bonds cholinesterase activity carboxylic ester hydrolase activity hydrolase activity catalytic activity cation binding acetylcholine binding ion binding binding small molecule binding collagen binding protein-containing complex binding extracellular matrix binding acetylcholinesterase activity hydrolase activity, acting on ester bonds cholinesterase activity carboxylic ester hydrolase activity hydrolase activity catalytic activity cation binding acetylcholine binding ion binding binding small molecule binding collagen binding protein-containing complex binding extracellular matrix binding protein binding laminin binding identical protein binding protein homodimerization activity protein dimerization activity serine hydrolase activity	localization import into cell receptor internalization transport cellular process receptor-mediated endocytosis endocytosis establishment of localization vesicle-mediated transport developmental process multicellular organismal process sensory organ development eye development animal organ development localization import into cell receptor internalization transport cellular process receptor-mediated endocytosis endocytosis establishment of localization vesicle-mediated transport developmental process multicellular organismal process sensory organ development eye development animal organ development retina development in camera-type eye visual system development multicellular organism development camera-type eye development anatomical structure development sensory system development system development osteoblast development cellular developmental process ossification cell differentiation cell development osteoblast differentiation acetate ester metabolic process catabolic process acetylcholine catabolic process acetylcholine metabolic process metabolic process regulation of biological process regulation of cellular process homeostatic process biological regulation positive regulation of biological process adaptive thermogenesis positive regulation of metabolic process multicellular organismal-level homeostasis regulation of cold-induced thermogenesis cold-induced thermogenesis temperature homeostasis positive regulation of cold-induced thermogenesis positive regulation of multicellular organismal process regulation of multicellular organismal process regulation of metabolic process positive regulation of cellular process cell adhesion cellular response to nitrogen compound cellular response to stimulus cellular response to acetylcholine response to stimulus signaling response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound cell communication cellular response to chemical stimulus synaptic signaling response to chemical response to acetylcholine signal transduction regulation of signaling regulation of receptor recycling regulation of macromolecule metabolic process receptor recycling receptor metabolic process macromolecule metabolic process	basement membrane cell periphery extracellular matrix cellular anatomical structure external encapsulating structure collagen-containing extracellular matrix cell surface plasma membrane membrane neuromuscular junction synapse cell junction side of membrane membrane-bounded organelle basement membrane cell periphery extracellular matrix cellular anatomical structure external encapsulating structure collagen-containing extracellular matrix cell surface plasma membrane membrane neuromuscular junction synapse cell junction side of membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm intracellular membrane-bounded organelle endomembrane system perinuclear region of cytoplasm extracellular region extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR050654	Acetylcholinesterase-related enzymes	Family
A, B, C, D	IPR000997	Cholinesterase	Family
A, B, C, D	IPR002018	Carboxylesterase, type B	Domain
A, B, C, D	IPR019819	Carboxylesterase type B, conserved site	Conserved Site
A, B, C, D	IPR019826	Carboxylesterase type B, active site	Active Site
A, B, C, D	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
A, B, C, D	IPR014788	Acetylcholinesterase, tetramerisation domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.