3CSL

Structure of the Serratia marcescens hemophore receptor HasR in complex with its hemophore HasA and heme

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
D	d3csld1	Alpha and beta proteins (a+b)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	(Serratia marcescens ) [TaxId: 615 ],	SCOPe (2.08)
D	d3csld2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Serratia marcescens ) [TaxId: 615 ],	SCOPe (2.08)
C	d3cslc1	Alpha and beta proteins (a+b)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	Heme-binding protein A (HasA)	(Serratia marcescens ) [TaxId: 615 ],	SCOPe (2.08)
C	d3cslc2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Serratia marcescens ) [TaxId: 615 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D	SCOP2B Superfamily	Heme-binding protein A (HasA)	8034957	3000777	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Heme-binding protein A (HasA)	8034957	3000777	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	TonB_dep_Rec	e3cslA1	A: beta barrels	X: Outer membrane meander beta-barrels	H: Porins	T: Ligand-gated protein channel	F: TonB_dep_Rec	ECOD (1.6)
A	Plug	e3cslA2	A: a+b complex topology	X: N0 domain in phage tail proteins and secretins-like	H: TonB-dependent receptor plug domain (From Topology)	T: TonB-dependent receptor plug domain	F: Plug	ECOD (1.6)
B	TonB_dep_Rec	e3cslB1	A: beta barrels	X: Outer membrane meander beta-barrels	H: Porins	T: Ligand-gated protein channel	F: TonB_dep_Rec	ECOD (1.6)
B	Plug	e3cslB2	A: a+b complex topology	X: N0 domain in phage tail proteins and secretins-like	H: TonB-dependent receptor plug domain (From Topology)	T: TonB-dependent receptor plug domain	F: Plug	ECOD (1.6)
D	HasA	e3cslD1	A: a+b two layers	X: Heme-binding protein A (HasA) (From Topology)	H: Heme-binding protein A (HasA) (From Topology)	T: Heme-binding protein A (HasA)	F: HasA	ECOD (1.6)
C	HasA	e3cslC1	A: a+b two layers	X: Heme-binding protein A (HasA) (From Topology)	H: Heme-binding protein A (HasA) (From Topology)	T: Heme-binding protein A (HasA)	F: HasA	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.170.130.10	Mainly Beta	Beta Complex	Ferric Hydroxamate Uptake Protein	Chain A, domain 1	CATH (4.3.0)
A	2.40.170.20	Mainly Beta	Beta Barrel	Maltoporin	Chain A	CATH (4.3.0)
B	2.170.130.10	Mainly Beta	Beta Complex	Ferric Hydroxamate Uptake Protein	Chain A, domain 1	CATH (4.3.0)
B	2.40.170.20	Mainly Beta	Beta Barrel	Maltoporin	Chain A	CATH (4.3.0)
D	3.30.1500.10	Alpha Beta	2-Layer Sandwich	Heme-binding Protein A	Chain: A	CATH (4.3.0)
C	3.30.1500.10	Alpha Beta	2-Layer Sandwich	Heme-binding Protein A	Chain: A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00593	TonB dependent receptor-like, beta-barrel (TonB_dep_Rec_b-barrel)	TonB dependent receptor-like, beta-barrel	This entry represents the beta-barrel domain of TonB-dependent receptors, such as BtuB, CirA, FatA, FcuT, FecA, FepA, among others [1].	Domain
A, B	PF07715	TonB-dependent Receptor Plug Domain (Plug)	TonB-dependent Receptor Plug Domain	The Plug domain has been shown to be an independently folding subunit of the TonB-dependent receptors ([1]). It acts as the channel gate, blocking the pore until the channel is bound by ligand. At this point it under goes conformational changes opens ...	The Plug domain has been shown to be an independently folding subunit of the TonB-dependent receptors ([1]). It acts as the channel gate, blocking the pore until the channel is bound by ligand. At this point it under goes conformational changes opens the channel.	Domain
C, D	PF06438	Heme-binding protein A (HasA) (HasA)	Heme-binding protein A (HasA)	Free iron is limited in vertebrate hosts, thus an alternative to siderophores has been developed by pathogenic bacteria to access host iron bound in protein complexes. HasA is a secreted hemophore that has the ability to obtain iron from hemoglobin. ...	Free iron is limited in vertebrate hosts, thus an alternative to siderophores has been developed by pathogenic bacteria to access host iron bound in protein complexes. HasA is a secreted hemophore that has the ability to obtain iron from hemoglobin. Once bound to HasA, the heme is shuttled to the receptor HasR, which releases the heme into the bacterium [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	HasR protein	heme transmembrane transporter activity transporter activity transmembrane transporter activity siderophore-iron transmembrane transporter activity siderophore uptake transmembrane transporter activity cation binding ion binding binding metal ion binding small molecule binding	-	cell periphery cell outer membrane cellular anatomical structure external encapsulating structure membrane cell envelope outer membrane
C, D	Hemophore HasA	cation binding ion binding binding metal ion binding small molecule binding	-	cellular anatomical structure extracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR039426	TonB-dependent receptor-like	Family
A, B	IPR037066	TonB-dependent receptor, plug domain superfamily	Homologous Superfamily
A, B	IPR011662	Secretin/TonB, short N-terminal domain	Domain
A, B	IPR010949	TonB-dependent haemoglobin/transferrin/lactoferrin receptor	Family
A, B	IPR011276	TonB-dependent haem/haemoglobin receptor	Family
A, B	IPR036942	TonB-dependent receptor-like, beta-barrel domain superfamily	Homologous Superfamily
A, B	IPR000531	TonB-dependent receptor-like, beta-barrel	Domain
A, B	IPR012910	TonB-dependent receptor, plug domain	Domain
C, D	IPR010495	Haem-binding HasA	Family
C, D	IPR036912	Haem-binding HasA superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B	OPM	Transmembrane	Beta-barrel transmembrane	Ligand-gated protein channels (n=22,S=24)	Outer Membrane Receptor (OMR)

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B	mpstruc	TRANSMEMBRANE PROTEINS: BETA-BARREL	Beta-Barrel Membrane Proteins: Monomeric/Dimeric	HasR heme-uptake receptor in complex with HasA hemophore and heme

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.