2WZS

Structure of the Family GH92 Inverting Mannosidase BT3990 from Bacteroides thetaiotaomicron VPI-5482 in complex with Mannoimidazole

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glyco_hydro_92_N	e2wzsA4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
A	Glyco_hydro_92_C	e2wzsA3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
A	Glyco_hydro_92_cat	e2wzsA2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
C	Glyco_hydro_92_N	e2wzsC4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
C	Glyco_hydro_92_C	e2wzsC3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
C	Glyco_hydro_92_cat	e2wzsC2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
E	Glyco_hydro_92_N	e2wzsE4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
E	Glyco_hydro_92_C	e2wzsE3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
E	Glyco_hydro_92_cat	e2wzsE2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
F	Glyco_hydro_92_N	e2wzsF4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
F	Glyco_hydro_92_C	e2wzsF3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
F	Glyco_hydro_92_cat	e2wzsF2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
G	Glyco_hydro_92_N	e2wzsG4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
G	Glyco_hydro_92_C	e2wzsG3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
G	Glyco_hydro_92_cat	e2wzsG2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
H	Glyco_hydro_92_N	e2wzsH4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
H	Glyco_hydro_92_C	e2wzsH3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
H	Glyco_hydro_92_cat	e2wzsH2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
B	Glyco_hydro_92_N	e2wzsB4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
B	Glyco_hydro_92_C	e2wzsB3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
B	Glyco_hydro_92_cat	e2wzsB2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)
D	Glyco_hydro_92_N	e2wzsD4	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_92_N	ECOD (1.6)
D	Glyco_hydro_92_C	e2wzsD3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_92_C	ECOD (1.6)
D	Glyco_hydro_92_cat	e2wzsD2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_92_cat	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
A	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
A	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
A	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
C	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
C	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
C	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
C	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
E	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
E	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
E	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
E	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
F	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
F	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
F	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
F	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
G	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
G	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
G	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
G	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
H	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
H	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
H	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
H	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
B	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
B	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
B	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
B	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)
D	2.70.98.10	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
D	3.30.2080.10	Alpha Beta	2-Layer Sandwich	GH92 mannosidase fold	GH92 mannosidase domain	CATH (4.3.0)
D	1.20.1050.60	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
D	1.20.1610.10	Mainly Alpha	Up-down Bundle	Glycosyl hydrolase family fold	alpha-1,2-mannosidases domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF07971	Glycosyl hydrolase family 92 catalytic domain (Glyco_hydro_92)	Glycosyl hydrolase family 92 catalytic domain	-	Repeat
A, B, C, D, E A, B, C, D, E, F, G, H	PF17678	Glycosyl hydrolase family 92 N-terminal domain (Glyco_hydro_92N)	Glycosyl hydrolase family 92 N-terminal domain	This domain is found at the N-terminus of family 92 glycosyl hydrolase proteins.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	PUTATIVE ALPHA-1,2-MANNOSIDASE	binding carbohydrate binding hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity hydrolase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cation binding ion binding metal ion binding small molecule binding	carbohydrate derivative catabolic process glycoprotein catabolic process catabolic process carbohydrate derivative metabolic process primary metabolic process protein catabolic process glycoprotein metabolic process macromolecule catabolic process metabolic process cellular process protein metabolic process macromolecule metabolic process carbohydrate metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR005887	Glycosyl hydrolase family 92, alpha-1,2-mannosidase, putative	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR008928	Six-hairpin glycosidase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR014718	Glycoside hydrolase-type carbohydrate-binding	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR012939	Glycosyl hydrolase family 92	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR050883	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR041371	Glycosyl hydrolase family 92 N-terminal domain	Domain