1RFF

Crystal structure of human Tyrosyl-DNA Phosphodiesterase complexed with vanadate, octapeptide KLNYYDPR, and tetranucleotide AGTT.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth A]	d1rffa1	Alpha and beta proteins (a+b)	Phospholipase D/nuclease	Phospholipase D/nuclease	Tyrosyl-DNA phosphodiesterase TDP1	Tyrosyl-DNA phosphodiesterase TDP1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D [auth B]	d1rffb1	Alpha and beta proteins (a+b)	Phospholipase D/nuclease	Phospholipase D/nuclease	Tyrosyl-DNA phosphodiesterase TDP1	Tyrosyl-DNA phosphodiesterase TDP1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth A]	SCOP2B Superfamily	Phospholipase D/nuclease-like	8037449	3000664	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Phospholipase D/nuclease-like	8037447	3000664	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	Phospholipase D/nuclease-like	8037449	3000664	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	Phospholipase D/nuclease-like	8037447	3000664	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth A]	Tyr-DNA_phospho_1st	e1rffA1	A: a+b three layers	X: Phospholipase D/nuclease (From Topology)	H: Phospholipase D/nuclease (From Topology)	T: Phospholipase D/nuclease	F: Tyr-DNA_phospho_1st	ECOD (1.6)
C [auth A]	Tyr-DNA_phospho_2nd	e1rffA2	A: a+b three layers	X: Phospholipase D/nuclease (From Topology)	H: Phospholipase D/nuclease (From Topology)	T: Phospholipase D/nuclease	F: Tyr-DNA_phospho_2nd	ECOD (1.6)
D [auth B]	Tyr-DNA_phospho_1st	e1rffB1	A: a+b three layers	X: Phospholipase D/nuclease (From Topology)	H: Phospholipase D/nuclease (From Topology)	T: Phospholipase D/nuclease	F: Tyr-DNA_phospho_1st	ECOD (1.6)
D [auth B]	Tyr-DNA_phospho_2nd	e1rffB2	A: a+b three layers	X: Phospholipase D/nuclease (From Topology)	H: Phospholipase D/nuclease (From Topology)	T: Phospholipase D/nuclease	F: Tyr-DNA_phospho_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth A]	3.30.870.10	Alpha Beta	2-Layer Sandwich	Endonuclease	Chain A	CATH (4.3.0)
D [auth B]	3.30.870.10	Alpha Beta	2-Layer Sandwich	Endonuclease	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth A], D [auth B]	PF06087	Tyrosyl-DNA phosphodiesterase (Tyr-DNA_phospho)	Tyrosyl-DNA phosphodiesterase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth D], B [auth F]	5'-D(APGPTPT)-3'	-	-	-
E [auth C], F [auth E]	Topoisomerase I-Derived Peptide	-	-	-
C [auth A], D [auth B]	Tyrosyl-DNA phosphodiesterase 1	nuclease activity hydrolase activity, acting on ester bonds hydrolase activity exonuclease activity catalytic activity phosphoric ester hydrolase activity tyrosyl-DNA phosphodiesterase activity 3'-tyrosyl-DNA phosphodiesterase activity catalytic activity, acting on DNA phosphoric diester hydrolase activity catalytic activity, acting on a nucleic acid DNA binding double-stranded DNA binding binding nuclease activity hydrolase activity, acting on ester bonds hydrolase activity exonuclease activity catalytic activity phosphoric ester hydrolase activity tyrosyl-DNA phosphodiesterase activity 3'-tyrosyl-DNA phosphodiesterase activity catalytic activity, acting on DNA phosphoric diester hydrolase activity catalytic activity, acting on a nucleic acid DNA binding double-stranded DNA binding binding organic cyclic compound binding nucleic acid binding single-stranded DNA binding	cellular response to stimulus response to stimulus single strand break repair primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair cellular response to stimulus response to stimulus single strand break repair primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair double-strand break repair	cell periphery cellular anatomical structure plasma membrane membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle cytoplasm nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm cell periphery cellular anatomical structure plasma membrane membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle cytoplasm nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C [auth A], D [auth B]	IPR010347	Tyrosyl-DNA phosphodiesterase I	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
C [auth A], D [auth B]	Pharos: Q9NUW8	spinocerebellar ataxia, autosomal recessive, with axonal neuropathy 1 Ataxias, Hereditary breast cancer epithelioid cell uveal melanoma medulloblastoma osteosarcoma atypical teratoid rhabdoid tumor Charcot-Marie-Tooth disease large cell medulloblastoma neurodegenerative disease psoriasis tuberculosis autosomal recessive cerebellar ataxia cutaneous lupus erythematosus lung cancer spinocerebellar ataxia, autosomal recessive, with axonal neuropathy 1 Ataxias, Hereditary breast cancer epithelioid cell uveal melanoma medulloblastoma osteosarcoma atypical teratoid rhabdoid tumor Charcot-Marie-Tooth disease large cell medulloblastoma neurodegenerative disease psoriasis tuberculosis autosomal recessive cerebellar ataxia cutaneous lupus erythematosus lung cancer non-small cell lung carcinoma spinocerebellar ataxia, autosomal recessive, with axonal neuropathy uveal melanoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.